The Protective Effects of Mouse Bone Marrow Mesenchymal Stem Cell Soup on Morphine-Induced Cell Death

Background: Bone Marrow Mesenchymal Stem Cell (BM-MSC) Soup shows potential as a therapeutic tool against degenerative diseases due to its diverse array of secreted factors. Morphine, commonly used for severe pain management in medical conditions such as cancer, has been reported to have varying effects on the proliferation and growth of cell lines. This study investigates the influence of mBM soup on morphine-induced cell death in PC12, U87, AGS, and MCF-7 cell lines. Methods: mBMSC condition medium, termed mBM Soup, was prepared after harvesting mBMSC cells. The cells were cultured in DMEM medium with periodic medium replacement. Upon reaching 80% confluency, the medium was switched to fresh DMEM free serum with 0.2% BSA. After 24 or 48 hours, the conditioned mediums were collected as mBM soup. Different treatments were applied to the cell lines, including morphine and various mBM soup combinations. Cell viability, morphological changes, and types of cell death were assessed using trypan blue staining, MTT assay, Hoescht/PI staining, and NO secretion analysis. Results: The viability assays indicated that mBM soup enhanced cell viability in a time-dependent manner (p<0.05) and reduced cell death rates (p<0.05). Discussion: The findings suggest that mBM soup can promote cell proliferation and inhibit morphine-induced cell death.