Synthesis, Characterization and Antimicrobial Activity of New Heterocyclic Compounds Incorporating Pyridazine Moiety

Coumarin and its derivatives have garnered significant interest in pharmacological and pharmaceutical research due to their wide range of biological activities. This study focuses on the synthesis of a series of novel heterocyclic compounds incorporating a pyridazine moiety, derived from 6-amino-4-methyl-2H-chromen-2-one. These compounds (2-14) were synthesized and evaluated for their antimicrobial properties. The structural integrity of the synthesized compounds was confirmed through spectral and elemental analysis. Additionally, four of these compounds were tested for anti-inflammatory activity using the carrageenan-induced hind paw edema method in rats. Results indicated that these coumarin derivatives not only decreased edema volume and serum levels of pro-inflammatory cytokines such as IL-6 and TNF-α but also enhanced the production of the anti-inflammatory cytokine IL-10, suggesting potential therapeutic applications.