Protective Effects of Mouse Bone Marrow Mesenchymal Stem Cell Soup on Staurosporine-Induced Cell Death in PC12 and U87 Cell Lines

Mouse bone marrow mesenchymal stem cell (mBMSC) soup is a promising therapeutic tool for neurodegenerative diseases due to its ease of procurement and potential for transplantation without rejection. This study evaluates the effects of mBMSC soup on staurosporine-induced cell death in PC12 and U87 cell lines. mBMSCs were cultured in DMEM medium, followed by incubation in serum-free medium with 0.2% bovine serum albumin for 24 and 48 hours. The conditioned medium, referred to as mBM soup, was collected. Six treatment groups were established: 1μM staurosporine, no staurosporine, mBM Soup 24h, mBM Soup 24h with 1μM staurosporine, mBM Soup 48h, and mBM Soup 48h with 1μM staurosporine. Results showed time-dependent increases in cell viability and decreases in cell death with mBM soup (p<0.05). Additionally, total neurite length and fraction of cell differentiation significantly improved compared to staurosporine-only treatment (p<0.05). These findings suggest mBM soup offers protective benefits against staurosporine-induced cell death, enhancing cell survival and neurite outgrowth.