Efficacy of Vitamin-C in Amelioration of Phorate-Induced Hepatotoxicity and Cytogenetic Damage in Mice

The present study evaluates the efficacy of vitamin C in combating phorate-induced oxidative stress and cellular damage in hepatic cells of mice, in vivo. Mice were simultaneously treated intra-peritoneally with phorate, either in a single (1mg/kg bw) or double (2mg/kg bw) dose, and with vitamin C (500mg/kg) for 3 and 7 days, respectively. Several biomarkers, such as chromosomal aberrations, micronuclei and sperm head anomalies, DNA fragmentation assay, and enzymatic parameters, namely, lipid peroxidation (LPO), total thiol content, aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT), along with histopathology of liver tissues, were studied. Overall results suggest that vitamin C showed amelioration in phorate-intoxicated mice, indicating its potential for therapeutic use in the management of phorate-induced toxicity.