Elucidating Cross-Kingdom Metabolic Pathway Interactions Using Integrated Multi-Omics

Understanding the intricate interactions within metabolic pathways across different biological kingdoms is pivotal for advancements in biotechnology and therapeutic development. This study aims to decipher the cross-kingdom metabolic communications by leveraging integrated multi-omics approaches. Utilizing advanced transcriptomic and metabolomic analyses, we explored the metabolic pathways in a plant-fungi-bacteria community. Key findings indicate a significant upregulation of the shikimate pathway in plants upon fungal interaction, with a 2.3-fold increase in chorismate synthase expression (p < 0.01). Concurrently, a novel bacterial metabolite was identified, potentially functioning as a signaling molecule, enhancing plant growth by 15% under stress conditions (p < 0.05). Our study reveals the potential for metabolic pathway engineering to optimize interspecies interactions for improved biomass production. These insights could propel advances in agriculture and bioenergy by designing synthetic communities with tailored metabolic interactions. Thus, deciphering these cross-kingdom metabolic dynamics not only enriches our fundamental understanding but also opens avenues for novel biotechnological applications.