Rania Abdelmonem Khattab, Dalia Galal Said
The aim of the current study is to analyze different parameters affecting the adherence process of bacterial species involved in eye infections due to contact lens usage. Ninety patients with clinical evidence of microbial keratitis who attended the outpatient clinic corneal unit department of the Research Institute of Ophthalmology, Cairo, Egypt, were investigated. Biofilm formation was determined using the biochemical methyl tetrazolium bromide (MTT) assay. Various factors influencing bacterial adhesion were examined. Thirty P. aeruginosa isolates and fifteen Staphylococcus aureus isolates exhibited adherence capability to contact lenses, with Pseudomonas aeruginosa showing a higher adherence rate compared to S. aureus. The study found inoculum size to be the most significant factor for P. aeruginosa adhesion, followed by incubation period and assay media.