Effects of Hormonal Combination and Vitamins on Shoot Induction in Solanum tuberosum (Agria)

Due to the sensitivity of potatoes to viruses, producing virus-free plants is crucial, as it reduces production costs and enhances performance. This study examines the effect of six hormonal and vitamin combinations on the main and secondary shoot lengths of Agria potatoes. Conducted between 2012 and 2014 under laboratory conditions, the experiment used a randomized complete block design with three replications. Multivariate analysis of variance indicated significant differences (p < 0.01) in all traits between treatments. The treatment with Pyridoxine 5 ppm, Thiamine 20 ppm, Biotin 10 ppm, and Cytokinin 5 ppm resulted in the longest main shoots, whereas the combination with Pyridoxine 5 ppm, Thiamine 5 ppm, Biotin 5 ppm, and Cytokinin 2 ppm produced the shortest. Thiamine 5 ppm was optimal for increasing both the minimum and mean secondary shoot lengths, while 10 ppm Biotin was best for extending side branches. To produce the greatest number of secondary shoots, Cytokinin 2 ppm was effective. For increased main shoot length, the combination of Pyridoxine 5 ppm, Thiamine 20 ppm, Biotin 10 ppm, and Cytokinin 5 ppm is recommended.