The Effects of Mouse Bone Marrow Mesenchymal Stem Cell Soup on Morphine-Induced Cell Death

Background: Bone Marrow Mesenchymal Stem Cell (BM-MSC) Soup is a promising tool for the treatment of degenerative diseases through various secreted factors. Morphine, used widely for severe pain management in conditions like cancer, has shown conflicting effects on cell proliferation and growth. This study aimed to determine the effect of BM-MSC Soup on morphine-induced cell death in PC12, U87, AGS, and MCF-7 cell lines. Methods: BM-MSCs were cultured and their conditioned medium, referred to as BM Soup, was prepared. Cells were treated with morphine and BM Soup (24h and 48h) to assess morphological changes using an inverted microscope, cell viability through trypan blue staining and MTT assay, cell death via Hoechst/PI staining, and NO secretion analysis. Results: Viability assays demonstrated that BM Soup increased cell viability in a time-dependent manner (p<0.05), while cell death decreased significantly (p<0.05). TNL and f% were notably higher compared to the morphine-only treatment (p<0.05). Discussion: The findings suggest that BM Soup enhances cell proliferation and growth, while mitigating morphine-induced cell death.