Development of Laser Light Observation Methods for Understanding the Cytoskeleton Components Relationship with Cell Life Processes: A Pilot Study

Methods for 3D imaging of cells and sub-cellular components, such as chromosomes, with and without additional laser irradiation, were developed and tested. The main goal of the study was to evaluate technologies for precise identification of changes in cellular components and compartments composed of biomolecules and intra-molecular structures, based on differences in their electron density. Unlike 2D images, 3D imaging allows for the observation of changes not only in volume rates due to absorption but also in the phase values of light passing through the observed objects. Despite the potential destructive effects of laser irradiation, this procedure could allow for the determination of the presence and localization of specific intra-cellular structures, depending on the exact location of the laser ray. Additionally, this approach provides initial information about the nature of bio-macromolecules within the composition of complex structures, which are important for basic cell functions and processes. These features could offer additional insights that might not be discernible when observing 2D objects.