Moringa oleifera-Based Diet Protects Against Nickel-Induced Hepatotoxicity in Rats

Context: Moringa oleifera leaves are renowned for their health-promoting benefits, particularly when incorporated into the diet. Objective: This study aimed to evaluate the protective effects of a Moringa oleifera-based diet against nickel (Ni)-induced hepatotoxicity in rats. Methods: Male rats were divided into six groups, receiving oral administration of 20 mg/kg body weight nickel sulfate in normal saline while being fed either a normal diet or Moringa oleifera-based diets over a 21-day period. Post-treatment, all animals were sacrificed under anesthesia 24 hours after the final administration. Results: Ni exposure significantly increased plasma activities of ALT, AST, and ALP, and altered lipid profiles, indicated by elevated TAG, CHOL, and LDL-C levels, and decreased HDL-C concentration. Additionally, Ni exposure increased plasma MDA levels and decreased GSH concentrations. Histopathological analysis revealed inflammation and cellular degeneration in the liver due to Ni exposure. Notably, the M. oleifera-based diet demonstrated hepatoprotective effects, evident from improved liver function indices, restored lipid profiles, and ameliorated cellular architecture. Conclusion: The findings support the health-promoting value of M. oleifera, highlighting its potential to mitigate hepatic injuries induced by toxic metal exposure.