In Vitro Investigation of Sideritis Libanotica Subsp. Linearis Labill. Plant’s Polar and Nonpolar Extracts on Human Plasma and Erythrocyte Acetylcholinesterase Enzyme

In this study, we investigated the effects of polar and nonpolar extracts from the Sideritis libanotica subsp. linearis Labill. plant on the acetylcholinesterase (AChE) enzyme, which is crucial for the human nervous system. Using methanol and hexane as solvents, we prepared extracts from both the plant and its roots. Our results demonstrated significant inhibition of AChE activity by the methanol extracts, with the plant and root extracts having I50 values of 1.066x10-3 mg/mL and 0.174x10-3 mg/mL, respectively. Conversely, hexane extracts showed negligible effects on plasma AChE. Inhibition assays revealed non-competitive inhibition in both plasma and erythrocyte environments, with no significant activity detected in erythrocyte AChE for hexane extracts. These findings suggest potential therapeutic applications of Sideritis libanotica extracts in modulating AChE activity, with implications for treating neurological disorders. Sideritis libanotica has traditionally been used for its health benefits, including immune system enhancement and alleviation of respiratory conditions.